Directed Electronics equalizer 2500 Podręcznik Użytkownika Pobierz pdf (Strona 63)

Load and Prime Reagents

HiSeq 2500 System User Guide

4 Slide the reagent rack out of the reagent compartment using the rack handle.

5 Remove the caps from each reagent tube and place the tube onto the rack in the

associated numbered position or matching label color.

Position

Reagent Description

HP8 or HP12 Index 1 (i7) Sequencing Primer Mix

HP3 Denaturation Solution

HT2 Wash Buffer

Table 6 Single-Indexed Run on a Single-Read Flow Cell or Paired-End Flow Cell

Position

Reagent Description

HP9 Index 2 (i5) SR Sequencing Primer Mix

HP8 or HP12 Index 1 (i7) Sequencing Primer Mix

HP3 Denaturation Solution

HT2 Wash Buffer

Table 7 Dual-Indexed Run on a Single-Read Flow Cell

Position

Reagent Description

RMR Resynthesis Mix

HP8 or HP12 Index 1 (i7) Sequencing Primer Mix

HP3 Denaturation Solution

HT2 Wash Buffer

Table 8 Dual-Indexed Run on a Paired-End Flow Cell

6 Place 15 ml conical tubes filled with 10 ml laboratory-grade water in unused rack

positions.

7 Slide the reagent rack into the reagent compartment, aligning the rack with the raised

guide on the floor of the compartment.

8 Lower the sippers into the tubes on the paired-end reagent rack as follows:

a Pull the handle towards you and then lower the handle while pulling it towards

you.

b Visually inspect the sippers to make sure that they do not bend as they lower into

the tubes.

c Release the handle into the slot on the bottom end of the groove.

9 Close the reagent compartment door.

1 2 ... 58 59 60 61 62 63 64 65 66 67 68 ... 153 154

Brak uwag

Directed Electronics equalizer 2500 Podręcznik Użytkownika Strona 63