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Troubleshooting
130
Part # 15035786 Rev. D
Stagger Runs on Flow Cell A and Flow Cell B
1 Wait for the run on the adjacent flow cell to begin a chemistry step, and then select
Pause. The Pause menu opens.
NOTE
Always pause the current run during a chemistry step opposed to an imaging step.
2 Select Normal Pause.
3 Wait for the software to complete the current chemistry step. The system is placed in a
safe state automatically.
4 After the adjacent run is paused, set up the new run.
5 After loading the new flow cell for the new run, close the compartment door.
6 Select Start to start the new sequencing run.
7 Select Resume on the adjacent flow cell to resume the paused run. The software
automatically controls chemistry and imaging processes on both flow cells.
- 2500 System 1
- User Guide 1
- Revision History 7
- Table of Contents 9
- Chapter 7 Troubleshooting 127 10
- Index 139 10
- Technical Assistance 143 10
- Overview 11
- HiSeq 2500 Components 13
- HiSeq 2500 Software 17
- Sample Sheet Overview 23
- Sequencing Consumables 25
- Perform a HiSeq v4 Run 27
- HiSeq v4 Sequencing Workflow 29
- Enter Run Parameters 31
- Load and Prime Reagents 37
- Perform a TruSeq v3 Run 51
- TruSeq v3 Sequencing Workflow 53
- Prepare Reagents for Read 2 75
- Load Reagents for Read 2 77
- Introduction 81
- Pre-Run Volume Check 85
- Perform a Rapid Run 100
- Load a Flow Cell 101
- Post-Run Procedures 107
- Unload and Weigh Reagents 109
- Perform a Maintenance Wash 111
- Perform a Water Wash 115
- Switch Sequencing Modes 117
- Shut Down the Instrument 119
- Part # 15035786 Rev. D 120
- Real-Time Analysis 121
- Real-Time Analysis Overview 123
- Monitor Run Metrics 125
- Real-Time Analysis Workflow 127
- Sequencing Output Files 131
- Output Folder Structure 133
- Tile Numbering 135
- Troubleshooting 137
- Possible Run Setup Problems 139
- Perform a Fluidics Check 141
- Stop and Resume a Run 143
- Primer Rehybridization 147
- HiSeq 2500 System User Guide 149
- Technical Assistance 153
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